How do you dispose of ethidium bromide?

Ethidium bromide is generally used in molecular biology laboratories. The concentration of Ethidium bromide is less than 0.1%, It discard by dry in a poly bag and autoclave. If the concentration of Ethidium bromide is equal or more than to 0.1%. it discard by dry in a Biohazard box and incineration.

How do you dispose of bromide?

Dispose of solid ethidium bromide through EHS as regular chemical waste. Aqueous solutions of ethidium bromide containing less than 1 microgram per milliliter (ug/ml) ethidium bromide and no other hazardous materials may be disposed down the drain with plenty of running water.

Is ethidium bromide hazardous waste?

All gels containing ethidium bromide are collected and disposed of through EH&S as chemical waste. The containers are provided by EH&S, must be labeled as “Non-hazardous Waste – Ethidium Bromide Gels & Debris” When the container is approximately 75 % full, submit an online Chemical Waste Pickup Request.

Is ethidium bromide a biohazard?

EtBr is a potent mutagen (may cause genetic damage), and moderately toxic after an acute exposure. EtBr is an irritant to the skin, eyes, mouth, and upper respiratory tract. It should be stored away from strong oxidizing agents in a cool, dry place, and the container must be kept undamaged and tightly closed.

Can ethidium bromide go down the drain?

Segregate and store your waste as follows: Solutions containing ethidium bromide: Small quantities of aqueous solutions containing < 10 µg/ml (10 ppm) may be flushed down the drain. Do not discard non-aqueous solutions down the drain. Do not mix solids or gels with solutions.

How bad is ethidium bromide?

Health and Safety EtBr is a potent mutagen (can cause genetic damage), and moderately toxic after an acute exposure. EtBr can be absorbed through skin, so it is important to avoid any direct contact with the chemical. The powder form is considered an irritant to the upper respiratory tract, eyes, and skin.

Is ethidium bromide flammable?

Not flammable or combustible. Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide. Wear self contained breathing apparatus for fire fighting if necessary. Wear respiratory protection.

Why is ethidium bromide mutagenic?

Because ethidium bromide can bind with DNA, it is highly toxic as a mutagen. It may potentially cause carcinogenic or teratogenic effects, although no scientific evidence showing either health effect has been found. Exposure routes of ethidium bromide are inhalation, ingestion, and skin absorption.

Why is ethidium bromide bad?

Although a highly sensitive stain, ethidium bromide is notoriously unsafe. Not only is it a very strong mutagen, it may also be a carcinogen or teratogenic. Its MSDS documents state that it is harmful if swallowed and very toxic by inhalation, as well as being irritating to the eyes, respiratory system and skin.

What happens if you inhale ethidium bromide?

Exposure routes of ethidium bromide are inhalation, ingestion, and skin absorption. An acute exposure to ethidium bromide causes irritation of the mouth, upper respiratory tract, skin, and eyes.