How are dendritic cells different from macrophages?

How are dendritic cells different from macrophages?

Until recently they have been regarded as relatively discrete cell types, with macrophages being a key component of the innate immune system while dendritic cells interface with the adaptive immune system and modulate immune responses.

Do macrophages express CD16?

Human monocytes express high levels of CD14 and low CD16, while macrophages express low CD14 and high CD16. Additionally, CD68 is a very classic marker for human macrophages. However, it is intracellular and you must permeabilize the cells if you’d like to do flow cytometric analysis.

Do dendritic cells express CD16?

CD16 is also expressed on granulocytes, tissue macrophages, and a subset of monocytes, eosinophils, and dendritic cells [5, 72, 73].

Do monocytes express CD16?

The most predominant of the three, termed “classical monocytes”, express high levels of CD14 on their surface, are devoid of surface CD16 and account for approximately 80% of the total monocyte population. The remaining 20% express CD16 and have been further classified into two subtypes.

Do dendritic cells come from macrophages?

Classifying mouse dendritic cells We propose that DCs should be classified as a separate lineage of mononuclear phagocytes on the basis of the fact that they arise from adult haematopoietic stem cell (HSC)-derived precursors that are distinct from the precursors of monocytes and macrophages (Fig. 1).

Do macrophages turn into dendritic cells?

During inflammation, Ly-6C+ monocytes give rise to monocyte-derived DCs, e.g. TNF and iNOS-producing dendritic cells (TipDC), inflammatory macrophages, and may contribute to myeloid-derived suppressor cells (MDSC) associated with tumors.

Do all macrophages express CD11b?

We find that CD206 is expressed at high levels by all tissue macrophage populations and by a subset of DC in some tissues. Expression of common myeloid markers on specific cell populations in non-lymphoid tissues.

What are the different types of macrophages?


Type of macrophage Location
Alveolar macrophage Lung alveoli
Kupffer cells Liver
Microglia Central nervous system
Splenic macrophages (marginal zone, metallophilic and red pulp macrophages) Spleen marginal zone, red and white pulp

Are dendritic cells CD11b+?

The CD11b+ classical dendritic cell subset In the muscle and lamina propria, CD11b+ cDCs arise from cDC-restricted precursors and monocytes, but markers to distinguish the two ontogenically distinct subsets differ between tissues.

Do macrophages express CD11c?

It is now recognized that several non-DC myeloid populations express CD11c, including alveolar macrophages and resident (Ly6C low) monocytes and that some macrophage populations express both CD11c and MHC class II [8, 9]. Similarly, CD11b has often been used as a marker for all myeloid cell types.

Is CD14 a monocyte marker?

Monocytes express typical blood monocyte markers, such as CD14, CD11b, CCR2, and CD16, but at extravascular sites they possess higher levels of CD141, CD11c, HLA-DR, and CCR7, indicating a tissue-imprinted phenotypic change that is reminiscent of DCs (111, 114).