How does EvaGreen dye work?

The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen™ dye is nonmutagenic and noncytotoxic by being completely impermeable to cell membranes (Figure 3), unlike SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer9.

Is SYBR Green in Intercalating dye?

Chemistry Developed and Real-Time RT-PCR Platforms The alternative is a nonsequence specific fluorescent intercalating ds DNA binding dye, e.g., SYBR Green I (Molecular Probes).

What kind of applications can EvaGreen dye be used for?

EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.

What can you do with EvaGreen 20x in water?

EvaGreen® Dye, 20X in Water. EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.

How long does EvaGreen dye last in PCR buffer?

Download the EvaGreen® Dye Safety Report for more information. EvaGreen® Dye is more convenient to handle because it has no detectable dye decomposition in PCR buffer at 95-100°C for 48 hours; is highly stable under either alkaline or acidic condition; and withstands repeated freeze-thaw cycles.

Which is better for PCR EvaGreen or SYBR Green?

Brighter and non-inhibitory: EvaGreen® is less inhibitory in the PCR reaction than SYBR® Green, permitting the use of saturating dye concentration for maximal signal and better high-resolution DNA melt analysis. Safer and more environmentally friendly: EvaGreen® dye is the first and only PCR dye to date designed to be environmentally safe.