What does MALDI matrix do?

What does MALDI matrix do?

MALDI is the abbreviation for “Matrix Assisted Laser Desorption/Ionization.” The sample for MALDI is uniformly mixed in a large quantity of matrix. The matrix absorbs the ultraviolet light (nitrogen laser light, wavelength 337 nm) and converts it to heat energy.

What is the function of the matrix in MALDI-TOF analysis?

The matrix performs two important functions: (1) it absorbs photon energy from the laser beam and transfers it into excitation energy of the solid system, and (2) it serves as a solvent for the analyte, so that the intermolecular forces are reduced and aggregation of the analyte molecules is held to a minimum.

What is the function of MALDI in determining the protein composition and structure?

The intact mass determination is basic and important for protein characterization, due to the correct molecular weight of a protein can indicate the intact structure. MALDI, a soft ionization technique, is suitable for proteins which tend to be fragile and fragment when ionized by other ionization methods.

Which matrix is used in MALDI technique?

MALDI-TOF MS is based on the use of a matrix that absorbs the energy of the laser irradiation and is, thus, crucial for the successful generation of ions. There are basically three different classes of matrices available: Classical organic matrices, such as benzoic acid or cinnamic acid derivatives.

What is MALDI technique?

In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation. First, the sample is mixed with a suitable matrix material and applied to a metal plate.

What is the full form of MALDI-TOF?

The matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid, accurate, and cost-effective method of microbial characterization and identification.

What makes a good MALDI matrix?

Your matrix should have a strong optical absorption in either the ultraviolet or infrared range, in order to rapidly and efficiently absorb the laser irradiation. The matrix needs to contain polar groups, which allow them to be used in aqueous solutions.

What makes a good maldi Matrix?