What is meant by slide agglutination test?

(ă-glū’ti-nā’shŭn) 1. The process by which suspended bacteria, cells, or other particles are caused to adhere and form clumps; similar to precipitation, but the particles are larger and are in suspension rather than being in solution.

How do you do a slide agglutination test on a dog?

To perform this test, place one drop of EDTA blood on a slide, and add one drop of saline solution (two drops for a cat). The purpose of the saline is to disperse any rouleaux formation as well as inflammatory proteins that may cause pseudo-agglutination.

What does slide test mean?

MACROSCOPIC SLIDE TEST A certain amount of concentrated killed antigen and patients serum are mixed on a plate, slide or card and allowed to react for a specified period, after which the presence of agglutination is determined by naked eye.

What does agglutination mean in dogs?

Agglutination. Agglutination refers to the phenomenon where red blood cells stick together in three-dimensional clumps.

What is slide agglutination test used for?

A rapid slide agglutination test has been developed for the identification of Neisseria gonorrhoeae that are primarily detected as oxidase-positive colonies in gonococcal cultures. The technique is based on the specific nonimmune reactivity between the Fc portion of immunoglobulin (Ig)G and staphylococcal protein A.

What is agglutination test used for?

The latex agglutination test is a laboratory method to check for certain antibodies or antigens in a variety of body fluids including saliva, urine, cerebrospinal fluid, or blood.

What is the process of agglutination?

Agglutination is defined as the formation of clumps of cells or inert particles by specific antibodies to surface antigenic components (direct agglutination) or to antigenic components adsorbed or chemically coupled to red cells or inert particles (passive hemagglutination and passive agglutination, respectively).

What are the types of agglutination?

There are two forms of agglutination. They are the active agglutination and the passive agglutination….Active agglutination

• biological technique.
• agglutination reaction.
• antigen.
• antiserum.
• sorption.
• spontaneous aggtination.
• immune agglutination.
• group agglutination.

What is agglutination test example?

Examples of agglutination in biology are clumping of cells such as bacteria (Widal test) or red blood cells (Blood grouping) in the presence of specific antibody. The antibody binds multiple antigen particles and joins them, creating a large lattice like complex which we can see with naked eye.

What is agglutination positive?

Agglutination of red blood cells in a given site indicates a positive identification of the blood antigens: in this case, A and Rh antigens for blood type A-positive.

How do you identify agglutination?

In agglutination tests, an antigen reacts with its corresponding antibody, resulting in visible clumping of bacterial cells. With latex agglutination tests, latex particles are coated with antibodies that agglutinate specific antigens and form a more easily visible precipitate.

What do you need to know about slide agglutination in dogs?

Slide Agglutination in Dogs. Share: Slide agglutination is a blood test that is sometimes used for dogs and other animals to screen for immune disease, particularly immune mediated hemolytic anemia. This immune disease causes the immune system to attack and destroy red blood cells.

How is the in saline agglutination test done?

The simplest in-clinic version of in-saline agglutination testing is the slide agglutination test, in which 4 to 10 drops of saline are added to 1 drop of anticoagulated blood on a microscope slide (FIGURE 5) and the sample is gently mixed by tilting the slide while watching for gross agglutination (speckles) (FIGURE 6).

How does a vet take blood for agglutination?

Your veterinarian draws a blood sample and places a drop of whole blood on a microscope slide. A drop of 0.9% saline is then added to the slide and mixed with the blood, and the slide is gently rocked. The blood is observed for agglutination, which looks like small clumps or peppering.

How is autoagglutination performed in a blood test?

This test is performed by placing a drop of EDTA-anticoagulated blood on a glass slide. Three or four drops of physiological saline then are placed on the drop of blood. A coverslip is placed over the mixture, which is observed unstained under a microscope using the 40× objective.