What is MethoCult?

MethoCult™ is a line of semi-solid methylcellulose-based media formulated to promote optimal growth and differentiation of hematopoietic progenitor cells in culture. A wide range of MethoCult™ media formulations are available for CFU assays with hematopoietic cells from human and mouse tissues.

What makes stem cells unique?

Stem cells provide new cells for the body as it grows, and replace specialised cells that are damaged or lost. They have two unique properties that enable them to do this: They can divide over and over again to produce new cells. As they divide, they can change into the other types of cell that make up the body.

What is the difference between CFU and BFU?

Whereas BFU-E cells are CD34-positive, CD36-negative, and CD71 low, CFU-E cells are CD34-negative and both CD36 and CD71 are positive. Thus, human BFU-E cells are CD45+GPA−IL-3R−CD34+CD36−CD71low, whereas CFU-E cells are CD45+GPA−IL-3R−CD34−CD36+CD71high.

What is a CFU assay?

The CFU assay is a hematopoietic functional assay, which is often used to measure the function or potency of hematopoietic progenitors present in stem cell products.

How is CFU calculated?

To find out the number of CFU/ ml in the original sample, the number of colony forming units on the countable plate is multiplied by 1/FDF. This takes into account all of the dilution of the original sample. For the example above, the countable plate had 200 colonies, so there were 200 CFU, and the FDF was 1/4000.

What is a myeloid stem cell?

They are derived from Hematopoietic stem cells. They differentiate into Erythrocyte progenitor cell (forms erythrocytes), Thrombocyte progenitor cell (forms platelets) and Granulocyte-Monocyte progenitor cell (forms monocytes, macrophages, neutrophils, basophils, eosinophils, dendritic cells).

What are the 3 types of stem cells?

Types of Stem Cells

• Embryonic stem cells.
• Tissue-specific stem cells.
• Mesenchymal stem cells.
• Induced pluripotent stem cells.

Which is derived from CFU-E?

CFU-E stands for Colony Forming Unit-Erythroid. It arises from CFU-GEMM (via BFU-E, which stands for “erythroid burst-forming units”) and gives rise to proerythroblasts.

What does BFU-E stand for?

Abbreviations: BFU-E, burst-forming units-erythroid; CFU-GM, colony forming units-granulocyte/macrophage; EpoR, erythropoietin.

How do you calculate the number of bacteria?

How to calculate the number of bacteria in a population

1. Example.
2. The mean division time for bacteria population A is 20 minutes.
3. In order to answer this, you can split the calculations into two sections.
4. If the bacteria grow for six hours, each bacterium will divide 3 times per hour × 6 hours = 18 times.

What kind of cells can MethoCult media be used for?

Select formulations can also be used with hematopoietic cells derived from human pluripotent stem cells (hPSCs) or from other species, specifically rats, non-human primates and dogs. Why Use MethoCult™ Media? STANDARDIZED AND QUALITY CONTROLLED.

How is MethoCult media used in CFU assays?

A wide range of MethoCult™ media formulations are available for CFU assays with hematopoietic cells from human and mouse tissues. Select formulations can also be used with hematopoietic cells derived from human pluripotent stem cells (hPSCs) or from other species, specifically rats, non-human primates and dogs. Why Use MethoCult™ Media?

What kind of cells can MethoCult h4434 classic support?

MethoCult™ H4434 Classic is formulated to support the optimal growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage and megakaryocyte progenitor cells (CFU-GEMM).

How are mouse bone marrow colonies imaged in MethoCult?

Mouse Bone Marrow Colonies Plated in MethoCult™ GF M3434, Imaged on STEMvision™ Instrument Ensure accurate and reproducible CFU assay results by using STEMvision™, a bench-top instrument and computer system for automated imaging and counting of hematopoietic colonies.