What is pre miR?

Pre-miR™ miRNA Precursor Molecules are small, chemically modified, double-stranded RNA molecules designed to mimic endogenous mature miRNAs. They enable detailed study of miRNA biological effects via gain-of-function experiments.

What are miRNA inhibitors?

miRNA inhibitors are natural or artificial RNA transcripts that sequestrate miRNAs and decrease or even eliminate their depressing effects. Competing endogenous RNAs (ceRNAs) can be viewed as natural and endogenous miRNA inhibitors.

What is the role of miRNA?

MicroRNAs (miRNAs) are a class of non-coding RNAs that play important roles in regulating gene expression. The majority of miRNAs are transcribed from DNA sequences into primary miRNAs and processed into precursor miRNAs, and finally mature miRNAs.

What is miRNA mimic and inhibitor?

We provide chemically synthesized RNA oligonucleotides optimized to mimic or inhibit miRNA molecules. Custom synthesized miRNA mimics and inhibitors are available with different labels and can be linked to cholesterol to increase cellular uptake.

Do miRNAs have a poly A tail?

Human Pri-miRNAs are polyadenylated and capped. A defining characteristic of almost all eukaryotic mRNAs is that they are terminally modified by addition of a 5′ 7-methyl guanylate (m7G) cap and a 3′ poly(A) tail.

How many miRNAs do humans have?

There are now over 2000 miRNAs that have been discovered in humans and it is believed that they collectively regulate one third of the genes in the genome. miRNAs have been linked to many human diseases and are being pursued as clinical diagnostics and as therapeutic targets.

How does miRNA inhibitor work?

The standard method for inhibiting microRNA (miRNA) function is by steric blocking, using an oligonucleotide that is perfectly complementary to the mature miRNA target. These inhibitors form a duplex with the miRNA guide strand and prevent the miRNA from binding to its intended target.

How do miRNA mimics work?

The miRNA mimic technology (miR-Mimic) is an innovative approach for gene silencing. Once introduced into cells, this RNA fragment, mimicking an endogenous miRNA, can bind specifically to its target gene and produce posttranscriptional repression, more specifically translational inhibition, of the gene.

How does miRNA bind to mRNA?

miRNAs (microRNAs) are short non-coding RNAs that regulate gene expression post-transcriptionally. They generally bind to the 3′-UTR (untranslated region) of their target mRNAs and repress protein production by destabilizing the mRNA and translational silencing.

Is miRNA a human?

How do you inhibit miRNA?

Why translation would be blocked if a micro RNA is bound to the mRNA molecule?

When the miRNA is perfectly complementary to the mRNA, the mRNA is often cut in two by an enzyme in the protein complex. When the miRNA is not perfectly complementary to the mRNA, the miRNA-protein complex may remain bound to the mRNA and block translation.