What is the disadvantage of streak plate technique?

Streak plating is a microbiology laboratory method that has two major disadvantages. Firstly, users will not be able to grow obligate anaerobes using this method. Secondly, only organisms that were viable in the original sample are able to be grown.

What are the disadvantages of pour plate technique over spread plate technique?

Disadvantages of Pour Plate Technique Colonies of different species within the agar appear similar — so it is difficult to detect contaminants. The reduced growth rate of obligate aerobes in the depth of the agar.

What are two advantages of the streak plate method?

Agar streak plates are an essential tool in microbiology. They allow bacteria and fungi to grow on a semi-solid surface to produce discrete colonies. These colonies can be used to help identify the organism, purify the strain free of contaminants, and produce a pure genetic clone.

What advantages does the streak plate method have over the pour plate method?

What advantage does the streak plate method have over the pour-plate method? The streak plate method does not require any additional media for dilution and only requires one plate for inoculation.

How can a streak plate become contaminated?

How can a streak plate become contaminated? If the loop is not sterilized. If you drop the plate.

What is the difference between pour plate and spread plate technique?

The key difference between Pour plate and Spread plate is that a known volume of the sample is spread on the surface of the agar medium in spread plate, while, in pour plate, a known volume of the sample is mixed with agar and then poured into a plate.

What is the difference between pour plate and spread plate method?

Which is better streak plate or pour plate?

The pour plate method of counting bacteria is more precise than the streak plate method, but, on the average, it will give a lower count.

Which is better streak plate or pour plate method?

What is the advantage of pour plate method?

Advantages of the Pour Plate Method The pour plate approach allows for more sensitivity, so is ideal for samples with lower bacterial numbers. It’s is also the best method for organisms requiring a microaerophilic environment. However, it can be a time consuming, labour intensive process, open to various errors.