What wavelength is rhodamine?
What wavelength is rhodamine?
Fluorochrome Peak Excitation and Emission Wavelengths
| Fluorochrome | Excitation Wavelength | Emission Wavelength |
|---|---|---|
| Rhodamine Green fluorophore | 502 | 527 |
| Rhodamine Red | 570 | 590 |
| Rhodamine WT | 530 | 555 |
| Rhodol Green fluorophore | 499 | 525 |
What wavelength of light is absorbed by fluorescein?
Fluorescein isothiocyanate (FITC), for example, will absorb light from 400-530 nm but absorbs most efficiently at its peak or excitation maximum of 490 nm wavelength.
At what wavelength does GFP fluorescence?
GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.
What is rhodamine used for?
Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a staining fluorescent dye.
What wavelength does Rhodamine B absorb?
Rhodamine B is a fluorescent compound with an excitation peak at 546 nm and an emission peak at 568 nm. It can be excited using a 561 nm laser paired with a 586/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta™.
What is rhodamine phalloidin?
Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC). Also available as a room-temperature-stable, ready-to-use solution: ActinRed 555 Ready Probes Reagent.
What wavelength is DAPI?
Specifications
| Color: | Blue |
|---|---|
| Detection Method: | Fluorescent |
| Dye Type: | Cell-Impermeant |
| Excitation Wavelength Range: | 358⁄461 |
| For Use With (Equipment): | Fluorescence Microscope |
What is excitation wavelength?
Excitation spectra. A fluorophore is excited most efficiently by light of a particular wavelength. This wavelength is the excitation maximum for the fluorophore. This wavelength is the emission maximum for that fluorophore. The excited fluorophore can also emit light at wavelengths near the emission maximum, as shown.
Is rhodamine sensitive to light?
Due to its more rigid structure than other organic dyes,rhodamine B is stable or exhibits an extremely slow degradation under visible light irradiation . Some dyes may be experienced to degradation upon exposure to sunlight especially in alkaline solutions. Rhodamine B is ozo dye, stronger than methylene blue dye.
What is the chemical formula of Rhodamine B?
C28H31ClN2O3
Rhodamine B/Formula
What is the melting temperature of rhodamine B?
The adsorption constant of dye to cells for Rhodamine B was 6.86 cal/mol (adsorptive affinity in Langmuir’s equation) and 2.86 (for the constant K in Freundlich’s equation). Melting temperature values for Rhodamine B calf thymus
Which is an example of rhodamine in water?
Rhodamine in water. Rhodamine /ˈroʊdəmiːn/ is a family of related chemical compounds, fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are used as dyes and as dye laser gain media. They are often used as a tracer dye within water to determine the rate and direction of flow and transport.
What kind of dye is Rhodamine B made of?
Rhodamine B is an organic chloride salt having N-[9-(2-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene]-N-ethylethanaminium as the counterion. An amphoteric dye commonly used as a fluorochrome. It has a role as a fluorochrome, a fluorescent probe and a histological dye. It is an organic chloride salt and a xanthene dye.
What can rhodamine 123 be used for in biotechnology?
Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Rhodamine 123 is also used in biochemistry to inhibit mitochondrion function.