Questions and answers

Does temperature affect isoelectric point?

Does temperature affect isoelectric point?

As shown in Fig. ​ 2, the isoelectric point of both proteins moves toward the left as the temperature changes from 25 to 100 °C.

How efficient is Joule heating?

As a heating technology, Joule heating has a coefficient of performance of 1.0, meaning that every joule of electrical energy supplied produces one joule of heat. In contrast, a heat pump can have a coefficient of more than 1.0 since it moves additional thermal energy from the environment to the heated item.

What affects isoelectric point?

The isoelectric point (pI) is the pH at which a particular molecule carries no net electrical charge. The net charge on the molecule is affected by the pH of its surrounding environment and can become more positive or negative due to the gain or loss of protons, respectively.

What is Pl of protein?

Isoelectric point, also called the pI of the protein, is the pH at which the net charge of the protein is zero. Isoelectric focusing is a separation technique which separates peptides according to their isoelectric point, or how acidic and basic their residues are.

What is the relationship between current and temperature?

Using an infrared thermometer, two German chemists have shown that the relationship between temperature and electric current is generally universal for CE, with a set increase in current producing a set increase in temperature.

What is meant by Joule heating effect?

Joule heating is the physical effect by which the pass of current through an electrical conductor produces thermal energy. This thermal energy is then evidenced through a rise in the conductor material temperature, thus the term “heating”.

Does resistance increase with temperature?

Heating a metal conductor makes it more difficult for electricity to flow through it. These collisions cause resistance and generate heat. Heating the metal conductor causes atoms to vibrate more, which in turn makes it more difficult for the electrons to flow, increasing resistance.

What is the importance of isoelectric point?

The isoelectric point is significant in protein purification because it represents the pH where solubility is typically minimal. Here, the protein isoelectric point signifies where mobility in an electro-focusing system is zero—and, in turn, the point where the protein will collect.

How does pH affect net charge of protein?

At high pH values, the net charge of most proteins is negative, where they bind to the positively-charged matrix in anion exchangers. When the environment is at a pH value equal to the protein’s pI, the net charge is zero, and the protein is not bound to any exchanger, and therefore, can be eluted out.

What type of protein is casein?

Like whey, casein is derived from milk Casein is 80% of the milk protein, while whey is 20%. Casein protein is digested slowly, while whey protein digested quickly. This is an important difference between these two popular dairy proteins. Like other animal proteins, casein is a complete protein source.