Is GlutaMAX same as glutamine?

Gibco GlutaMAX supplement is an improved cell culture supplement that can be used as a direct substitute for L-glutamine in your cell culture media. GlutaMAX supplement is a dipeptide, L-alanine-L-glutamine, which is more stable in aqueous solutions and does not spontaneously degrade.

Does Dmem have L-glutamine?

L-glutamine in DMEM/F12 Nutrient Mixture is 2.5 mM. L-glutamine in DMEM, GMEM, IMDM and H-Y medium is 4 mM. IMDM is often used as a starting formulation for proprietary hybridoma cell culture media. Hybridoma cells grow better in concentrations of L-glutamine that are above the average levels found in media.

What does GlutaMAX mean?

Glutamax™ is a dipeptide compound (L-alanine-L-glutamine) that does not spontaneously degrade and is commonly used as a cell culture media supplement.

What is Dmem GlutaMAX?

DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM contains no proteins, lipids, or growth factors. DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Can cells survive without glutamine?

Glutamine is a conditionally essential amino acid that continues to function at very low concentrations to support the growth of nontransformed cells, but transformed cells require much larger concentrations to survive.

Should you take L-glutamine?

While L-glutamine supplementation is usually considered safe for most people, there are some who should avoid it. People with kidney disease, liver disease or Reye’s syndrome, a severe condition that can cause swelling of the liver and brain, should avoid taking L-glutamine supplements.

What is in DMEM media?

DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.

What is RPMI used for?

RPMI is used when laboratory testing requires fresh, unfixed specimens such as flow cytometry as well as some FISH, molecular and cytogenetics tests. 2 ) Why is it pink/red? It has a phenol red pH indicator that will turn from pink to yellow indicating contamination or exposure to air.