What is a gel documentation system?
What is a gel documentation system?
Gel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis.
How does a gel doc work?
Generally, a gel doc includes an ultraviolet (UV) light transilluminator, a hood or a darkroom to shield external light sources and protect the user from UV exposure, and a CMOS camera for image capturing.
What is electrophoresis and gel documentation?
A: Electrophoresis is a method of analysis used to separate and characterize proteins, nucleic acids, and subcellular sized particles, such as viruses and tiny organelles. A: Gel documentation allows for particles to be characterized by both size and charge due to the sieving effect that occurs from the gel solution.
What is Transilluminator with documentation?
Description. The bench-top UV transilluminator series offers the researcher uniform and intense sources of ultraviolet light (radiation) with a compact footprint. Their special design emits either one or dual intensity excitation UV wavelengths for back-illumination of transparent fluorescent materials.
What is the purpose of SDS PAGE?
SDS-PAGE is an analytical technique to separate proteins based on their molecular weight.
What is a UV Transilluminator?
An ultra-violet (UV) transilluminator is a standard piece of equipment used in life science laboratories for visualization of target DNAs and proteins. The key application for a UV transilluminator is for visualization of DNA and protein agarose and polyacrylamide gels after electrophoresis.
What is the purpose of gel documentation system?
Gel documentation, or gel imaging, systems are used to record and measure labeled nucleic acid and protein in various types of media such as agarose, acrylamide or cellulose.
Why do we do gel electrophoresis?
Gel electrophoresis is used to separate macromolecules like DNA, RNA and proteins. DNA fragments are separated according to their size. Proteins can be separated according to their size and their charge (different proteins have different charges).
What is the principle of UV Transilluminator?
An ultra-violet (UV) transilluminator is a standard piece of equipment used in life science laboratories for visualization of target DNAs and proteins. The UV transilluminator works by emitting high levels of UV radiation through the viewing surface.
What is the function of Transilluminator?
A transilluminator uses an intense source of UV radiation to make visible the fluorescent marker used in electrophoresis gels. The gel is placed on the transilluminator window and is illuminated from below. Ultraviolet radiation is hazardous to both skin and eyes.
What is the basic principle of SDS-PAGE?
The principle of SDS-PAGE states that a charged molecule migrates to the electrode with the opposite sign when placed in an electric field. The separation of the charged molecules depends upon the relative mobility of charged species. The smaller molecules migrate faster due to less resistance during electrophoresis.
Is SDS a detergent?
This lauryl-grade sodium dodecyl sulfate (SDS) is a popular anionic detergent for routine protein electrophoresis and cell lysis methods.
What kind of light does a gel doc use?
Generally, a gel doc includes an ultraviolet (UV) light transilluminator, a hood or a darkroom to shield external light sources and protect the user from UV exposure, and a CMOS camera for image capturing. The main manufacturers of Gel documentation systems are Bio Rad, Azure Biosystems, Bioolympics, Syngene, Vilber Lourmat, UVItec and Aplegen.
Which is the best system for gel documentation?
It is the only benchtop system that seamlessly integrates DNA sample separation and gel imaging into one workflow. The E-Gel Imager System offers a safe, affordable, personal gel imaging system for documenting and analyzing agarose gels and E-Gel cassettes. The system has a compact design with a small footprint.
Which is the best UV transilluminator for gel documentation?
For ; SYBR Green, SYBR Gold, SYPRO Ruby, SYPRO Orange・FIT, GFP. 465nm blue LED will be a powerful tool for ecological fluorescence documentation. Together with such high-performance digital cameras as Canon PowerShot G11/G12, documentation comes in reasonably handy!!
Which is Thermo Fisher Scientific gel documentation system?
Whether simplicity, price, or maximum performance is a priority, our complete portfolio of gel documentation systems or gel imaging systems offers you options to suit the needs of your laboratory.