Questions and answers

What is an organotypic model?

What is an organotypic model?

Organotypic models are 3D in vitro representations of an in vivo environment. Their complexity can range from an epidermal replica to the establishment of a cancer microenvironment. These models have been used for many years, in an attempt to mimic the structure and function of cells and tissues found inside the body.

What is an organotypic culture?

Organotypic culture is defined as the culture of an organ collected from an organism. It is one method allowing the culture of complex tissues or organs. Several protocols have already been developed like agar methods, watch glass protocols, organ culture in media, and filter well inserts.

What are cell lines?

Introduction. Cell lines are cultures of animal cells that can be propagated repeatedly and sometimes indefinitely. They arise from primary cell cultures. Primary cultures are initiated directly from the cells, tissues, or organs of animals and are typically used in experiments within a few days.

What is primary culture in microbiology?

Primary culture refers to the stage of the culture after the cells are isolated from the tissue and proliferated under the appropriate conditions until they occupy all of the available substrate (i.e., reach confluence). After the first subculture, the primary culture becomes known as a cell line or subclone.

What is an organotypic slice?

Organotypic brain slice cultures are a well-established technique. This relatively simple method cultures brain tissue explants from neonatal mice/rats on a porous membrane insert that acts as an interface between the humidified incubator atmosphere and the culture medium that provides nutrition 6.

Can cell lines become immortal?

Changes from nonimmortal origins. While immortalised cell lines often originate from a well-known tissue type, they have undergone significant mutations to become immortal.

What are the primary cell types?

There are only two main types of cells: prokaryotic and eukaryotic. Prokaryotic cells lack a nucleus and other membrane-bound organelles.

What are the two types of cell lines?

Understanding Types of Cell Lines

  • Frog cell lines.
  • Hamster cell lines.
  • Mouse cell lines.
  • Rat cell lines.
  • Dog cell lines.

How are cell lines named?

Subclones are named by their parental cell line, followed by a hyphen and the subclone number (Table 3). Furthermore, the standardised name provides information about a cell line’s provenance as well as allowing traceability and comparability of the nomenclature-specific information.