Where is camk2 expressed?

hippocampus
Overall, CaMKIIα -GFP expression is strong and highly heterogeneous, with the dentate gyrus of the hippocampus as the most abundantly expressed region. In the hippocampus, around 70% of granule and pyramidal neurons expressed strong GFP.

Is CaMKII a gene?

GeneCards Summary for CAMK2A Gene CAMK2A (Calcium/Calmodulin Dependent Protein Kinase II Alpha) is a Protein Coding gene. Diseases associated with CAMK2A include Mental Retardation, Autosomal Recessive 63 and Mental Retardation, Autosomal Dominant 53.

Is CaMKII in all neurons?

All four CaMKII isoforms (α, β, δ, and γ) have been localized in brain (Takaishi et al., 1992). In addition, CaMKIIδ and CaMKIIγ have been localized within multiple tissues outside the central nervous system (Tobimatsu & Fujisawa, 1989).

What is CaMKII promoter?

About CaMKII Promoter: Calcium / Calmodulin-dependent protein Kinase II (CaMKII) is a serine/threonine-specific protein kinase that is regulated by the Ca2+/calmodulin complex. CaMKII promoter were used for transgene expression to obtain preferential gene expression in CNS for the excitatory neuron populations.

What is camk2?

a CaMKII enhances AMPA receptor (AMPAR) conductance in isolated CA1 pyramidal cells of wild-type mice but not in knockin mice in which phosphorylation of the AMPAR subunit glutamate receptor 1 (GluR1) on S831 or both S831 and S845 is prevented by mutation of the serine residues to alanine residues.

What is the function of calcineurin?

Calcineurin plays a key role in regulating the transcription factor NF-AT during T-cell activation, and in mediating responses of microorganisms to cation stress.

What is the role of CaMKII in LTP?

CaMKII subsequently translocates to the synapse, where it binds to NMDA-type glutamate receptors and produces potentiation by phosphorylating principal and auxiliary subunits of AMPA-type glutamate receptors. These processes are all localized to stimulated spines and account for the synapse-specificity of LTP.

What is hSyn promoter?

This biomodule encodes for Human synapsin 1 gene promoter (hSyn). hSyn confers highly neuron-specific long-term transgene expression in the adult rat brain. The AAV collection was developed by Sirion Biotech AG and is made available for research purposes only.

What is ef1a promoter?

EF1 promoter (Ubiquitous) in pDRIVE expression plasmid The EF-1 alpha gene encoding elongation factor-1 alpha is an enzyme which catalyzes the GTP-dependent binding of aminoacyl-tRNA to ribosomes. EF-1α is one of the most abundant proteins in eukaryotic cells and is expressed in almost all kinds of mammalian cells.

What does CaMKII stand for?

CaMKII

What happens when CaMKII is activated?

In summary, the available data point to the following model: activated CaMKII diffuses to the synapse and binds to NR2B, an event that is necessary for LTP (FIG. 3). Other binding partners may further enhance the amount of CaMKII in the PSD and spine head.

What does CAMK2A stand for in protein kinase II?

(Camk2a = calcium-calmodulin-dependent protein kinase II, alpha subunit; Prkaca = cAMP-dependent protein kinase, alpha-catalytic subunit; Neto2 = neuropilin and tolloid-like protein 2; Gluk1 = kainate receptor Gluk1)

Where is the CAMK2A promoter located in the hippocampus?

CaMKIIα-Cre line T29-1 transgenic mice have the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter driving Cre recombinase expression in the forebrain, including the CA1 pyramidal cell layer of the hippocampus.

Is the camk2a-creert2 transgene viable in mice?

Mice hemizygous for the Camk2a-CreERT2 transgene are viable and fertile, with expression of CreER T2 fusion protein (CreERT2 fusion protein) directed to neural populations by the mouse calcium/calmodulin-dependent protein kinase II alpha promoter region.

What is the role of CaMKII in AMPK activation?

CAMKII plays a key role in mediating caloric restriction-induced AMPK activation through increased production of hydrogen peroxide. Immunogold staining and electron microscopy of heart vibrosections was performed to localize Kv4.2/Kv4.3 and Ca (2+)/calmodulin-dependent protein kinase II (CaMKII) proteins.