Questions and answers

Which slide is used for checking WBC?

Which slide is used for checking WBC?

For a differential WBC count, an oil-immersion objective with around 100x magnification (1.4NA) is used. However, at these high magnification powers, the FOV is very small, which necessitates mechanical scanning of the glass slide during the counting process.

How can you identify white blood cells under a microscope?

Microscopy. Given that all white blood cells are over 5 micrometers in diameter, they are large enough to be seen using a typical optical microscope (compound microscope). Staining with Leishman’s stain makes it possible to not only easily identify different types of leukocytes, but also count them.

What is WBC Slideshare?

COMPONENTS OF CBC  Hematocrit (Hct)  Hemoglobin (Hbg)  Red blood cell count (RBC or erythrocyte count)  RBC INDICES  Mean corpuscular volume (MCV)  Mean corpuscular hemoglobin (MCH)  Mean corpuscular hemoglobin concentration (MCHC)  White blood cell count (WBC or Leukocyte count)  WBC differential count  …

How do you identify white blood cell types?

WBCs are divided into two main groups based on cytoplasmic appearance: agranular leukocytes (lymphocytes and monocytes that have relatively clear cytoplasm) and granular leukocytes (neutrophils, eosinophils, and basophils whose cytoplasm is filled with granules.

How is WBC count calculated?


  1. One large area is 1 x 1 mm, and the depth is 0.1 mm.
  2. Total area counted in 4 large squares = 4 x 1 x o. 1 = 0.4 µL (4/10).
  3. Y x 10/4 is the total WBC in the cell in 1 µL.
  4. Now dilution is 1:20.
  5. Number of WBC in 1µL = Y x 10 x 20/4 = Y x 50 = Total WBC count.
  6. Total TLC = counted cells (Y) x 50 = TLC/cmm.

Which is the smallest WBC?

Lymphocytes are agranular leukocytes which form from the lymphoid cell line within the bone marrow. They respond to viral infections and are the smallest leukocytes, with a diameter of 6-15µm.

Why white blood cells are white in Colour?

Explanation: Hence the name, white blood cells are white. Due to the constant fighting of illnesses, white blood cells are always consuming bacteria in a process called phagocytosis. During this process, they move around the bacteria and engulf it, like the diagram below.

How do you perform a CBC?

How Is a CBC Done?

  1. clean the skin.
  2. put an elastic band (tourniquet) above the area to get the veins to swell with blood.
  3. insert a needle into a vein (usually in the arm inside of the elbow or on the back of the hand)
  4. pull the blood sample into a vial or syringe.
  5. take off the elastic band and remove the needle from the vein.

What kills white blood cells?

Cancer treatments such as chemotherapy or radiation therapy can destroy white blood cells and leave you at risk for infection. Infection. A higher-than-normal white blood cell count usually means you have some type of infection. White blood cells are multiplying to destroy the bacteria or virus.