What is a co-culture of cells?

At the basic level, a co-culture is a cell cultivation set-up, in which two or more different populations of cells are grown with some degree of contact between them.

What are the steps of cell culture?

Basic Cell Culture Processes and Procedures

• Thawing.
• Cell Seeding.
• Cell Observation.
• Cell Observation.
• Medium Exchange.
• Passage.
• Cell Observation.
• Cell Detachment.

What are some key requirements for cell cultures?

Culture Conditions

• A substrate or medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals)
• Growth factors.
• Hormones.
• Gases (O2, CO2)
• A regulated physico-chemical environment (pH, osmotic pressure, temperature)

What is a co-culture assay?

The monitoring of network dynamics in real time using live cell imaging and the interrogation of the angiogenic network using high content imaging allows a full assessment of the use of agents aimed at modulating blood vessel formation and growth. …

What are examples of co-cultures?

Some examples of co-cultures include: Jewish-Americans, Deaf Americans, African- Americans, senior citizens, gays and lesbians, even gang membership.  High / Low context, Human nature, Noncontact / contact culture, etc.

What’s the difference between co-culture and subculture?

Some examples of subcultures are LGBT, bodybuilders, nudists, hip hop, grunge. On the other hand, countercultures are groups of people who differ in certain ways from the dominant culture and whose norms and values may be incompatible with it. Some examples are: Englightenment, Suffragettes, Romanticism.

What are the different types of cell lines?

Understanding Types of Cell Lines

• Frog cell lines.
• Hamster cell lines.
• Mouse cell lines.
• Rat cell lines.
• Dog cell lines.

What types of cells are used for cell culture?

There are two types of primary cells: Adherent cells – Also referred to as anchorage dependent cells, these are the type of cells that require attachment for growth….There are three major types of cell culture, which include:

• Primary cell culture.
• Secondary cell culture, and.
• Cell line.

What is a killing assay?

Radioactive isotope 51chromium (51Cr) or tritium (3H) killing assays are the traditional methods to assess virus-specific T cell-mediated cytotoxicity (Brunner et al., 1968; Usharauli et al., 2006). They are based on generating infected target cell populations and labeling them with 51Cr or 3H.

What is co-culture give five examples of co-cultures?

Remember, a co-culture is a subgroup one can belong to (such as religion, gender, age, physical abilities, country of origin, sexual orientation, etc.) Some examples of co-cultures include: Jewish-Americans, Deaf Americans, African- Americans, senior citizens, gays and lesbians, even gang membership.

What are the similarities of culture and co-culture?

Co-cultures are subsets of larger cultures, sharing similar features with the larger cultures of which they are a part. Cultures define a group of people sharing a common language, religion, notions about community, etc.; co-cultures are more complex.

Can anyone suggest a ” classic ” co-culture protocol?

If u guys want to study the effect on cell proliferation, u guys can label one cell line with GFP and other cell line with RFP and then study the proliferation using FACS analysis. Thank you! Sandip, second url ( www.gbo.com/fileadmin/user…/F074059_AN_Co-Culture.pdf) is not working unfortunately…

What are the safety guidelines for cell culture?

The following is a general guideline for culturing of cell lines. All cell culture must be undertaken in microbiological safety cabinet using aseptic technique to ensure sterility. 1. Hood regulations (a) Close hood sash to proper position to maintain laminar air flow (b) Avoid cluttering 2.

What can cell culture be used to study?

Cell Culture Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, and mutagenesis and carcinogenesis.

What is the protocol for microglia and neuron culture?

Culture medium was prepared as described by the manufacturer’s protocol. For microglia culture, DMEM is supplemented with 10% of FBS and Penicillin-Streptomycin (1:100 dilution). For neuron culture, Neurobasal Medium is modified with B27 supplements (1: 50 dilution) and 0.5 mM L-glutamine.